@Article{ZimbardiCCAMRAJF:2016:PoApDy,
author = "Zimbardi, Ana and Camargo, Priscila de F{\'a}tima and Carli,
Sibeli and Aquino Neto, Sidney and Meleiro, Luana P. and Rosa,
Jos{\'e} C. and Andrade, Adalgisa R. de and Jorge, Jo{\~a}o A.
and Furriel, Rosa P. M.",
affiliation = "{Universidade de S{\~a}o Paulo (USP)} and {Instituto Nacional de
Pesquisas Espaciais (INPE)} and {Universidade de S{\~a}o Paulo
(USP)} and {Universidade de S{\~a}o Paulo (USP)} and
{Universidade de S{\~a}o Paulo (USP)} and {Universidade de
S{\~a}o Paulo (USP)} and {Universidade de S{\~a}o Paulo (USP)}
and {Universidade de S{\~a}o Paulo (USP)} and {Universidade de
S{\~a}o Paulo (USP)}",
title = "A high redox potential laccase from pycnoporus sanguineus RP15:
potential application for dye decolorization",
journal = "International Journal of Molecular Sciences",
year = "2016",
volume = "17",
number = "5",
pages = "672",
keywords = "Pycnoporus sanguineus, solid state fermentation, response surface
methodology, laccase, purification, characterization,
decolorization, remazol brilliant blue R, Reactive blue 4,
bromophenol blue.",
abstract = "Laccase production by Pycnoporus sanguineus RP15 grown in wheat
bran and corncob under solid-state fermentation was optimized by
response surface methodology using a Central Composite Rotational
Design. A laccase (Lacps1) was purified and characterized and the
potential of the pure Lacps1 and the crude culture extract for
synthetic dye decolorization was evaluated. At optimal conditions
(eight days, 26 \˝C, 18% (w/w) milled corncob, 0.8% (w/w)
NH4Cl and 50 mmol¨ L ´1 CuSO4, initial moisture 4.1 mL¨ g ´1 ),
the laccase activity reached 138.6 \˘ 13.2 U¨ g ´1 . Lacps1
was a monomeric glycoprotein (67 kDa, 24% carbohydrate). Optimum
pH and temperature for the oxidation of
2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) were 4.4
and 74.4 \˝C, respectively. Lacps1 was stable at pH 3.08.0,
and after two hours at 5560 \˝C, presenting high redox
potential (0.747 V vs. NHE). ABTS was oxidized with an apparent
affinity constant of 147.0 \˘ 6.4 µmol¨ L ´1 , maximum
velocity of 413.4 \˘ 21.2 U¨ mg´1 and catalytic efficiency
of 3140.1 \˘ 149.6 L¨ mmol´1 ¨ s ´1 . The maximum
decolorization percentages of bromophenol blue (BPB), remazol
brilliant blue R and reactive blue 4 (RB4), at 25 or 40 \˝C
without redox mediators, reached 90%, 80% and 60%, respectively,
using either pure Lacps1 or the crude extract. This is the first
study of the decolorization of BPB and RB4 by a P. sanguineus
laccase. The data suggested good potential for treatment of
industrial dye-containing effluents.",
doi = "10.3390/ijms17050672",
url = "http://dx.doi.org/10.3390/ijms17050672",
issn = "1422-0067",
label = "lattes: 4809959472520732 2 ZimbardiCCAMRAJF:2016:PoApDy",
language = "en",
targetfile = "zimbardi_high.pdf",
urlaccessdate = "28 abr. 2024"
}